Coding
MtrB

Part:BBa_K1769005

Designed by: Ho-Tsang Tsai   Group: iGEM15_NYMU-Taipei   (2015-09-15)

MtrB Outer Membrane Structural Protein

Usage and Biology

MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. Two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None